DEPTOR/DEPDC6 (D9F5) Rabbit mAb

• 产品编号：CST-11816S      品牌：CST       原厂货号：11816S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

DEPTOR/DEPDC6 (D9F5)Rabbit mAb兔单抗可以识别内源性总的DEPTOR/DEPDC6 (D9F5)蛋白。

单克隆抗体由合成肽段免疫动物产生，该肽段与人DEPTOR/DEPDC6的Leu113邻近残基序列一致。

DEPTOR/DEPDC6是mTORC1和mTORC2复合物的组分（1）。它能够与mTOR结合，抑制mTORC1和mTORC2激酶活性（1）。mTOR，与casein激酶I一起，能够在生长信号存在的条件下磷酸化DEPTOR/DEPDC6，这会导致DEPTOR/DEPDC6的降解（2）。研究表明过表达DEPTOR/DEPDC6的转基因小鼠白色脂肪组织增多，在肥胖人群白色脂肪组织中DEPTOR/DEPDC6表达量上升（3）。此外，DEPTOR/DEPDC6的表达能够在小鼠脂肪细胞分化过程中被激活（3）。同时这些发现提示DEPTOR/DEPDC6调控脂肪形成（3）。

1. Peterson, T.R. et al. (2009) Cell 137, 873-86.
2. Gao, D. et al. (2011) Mol Cell 44, 290-303.
3. Laplante, M. et al. (2012) Cell Metab 16, 202-12.

原厂资料：

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin)

Specificity / Sensitivity

DEPTOR/DEPDC6 (D9F5) Rabbit mAb recognizes endogenous levels of total DEPTOR/DEPDC6 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide

Background

DEPTOR/DEPDC6 is a component of both mTORC1 and mTORC2 complexes (1). It interacts with mTOR and inhibits mTORC1 and mTORC2 kinase activities (1). mTOR, along with casein kinase I, phosphorylates DEPTOR/DEPDC6 in the presence of growth signals, which leads to the degradation of DEPTOR/DEPDC6 (2). Research studies have shown that transgenic mice overexpressing DEPTOR/DEPDC6 have more white adipose tissue, and DEPTOR/DEPDC6 expression levels increase in the white adipose tissue of obese humans (3). Furthermore, the expression of DEPTOR/DEPDC6 is induced during mouse adipocyte differentiation (3). Together these findings suggest that DEPTOR/DEPDC6 regulates adipogenesis (3).

corresponding to residues surrounding Leu113 of human DEPTOR/DEPDC6 protein.

1. Peterson, T.R. et al. (2009) Cell 137, 873-86.
2. Gao, D. et al. (2011) Mol Cell 44, 290-303.
3. Laplante, M. et al. (2012) Cell Metab 16, 202-12.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
*Species cross-reactivity is determined by western blot.
** Anti-rabbit secondary antibodies must be used to
detect this antibody.
Recommended Antibody Dilutions:
Western blotting 1:1000
Immunoprecipitation 1:50
Immunohistochemistry (Paraffin) 1:1000†
 Unmasking buffer: Citrate
 Antibody diluent: SignalStain® Antibody Diluent #8112
 Detection reagent: SignalStain® Boost (HRP, Rabbit) #8114
†Optimal IHC dilutions determined using SignalStain® Boost
IHC Detection Reagent.