ATF-4 (D4B8) Rabbit mAb

• 产品编号：CST-11815S      品牌：CST       原厂货号：11815S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

ATF-4 (D4B8) Rabbit mAb能够检测内源性ATF-4总蛋白水平。

该单克隆抗体是由合成的人源 ATF-4蛋白C末端肽段免疫动物而制备的。

ATF-4是激活转录因子/ cAMP反应元件结合蛋白家族成员，在PERK和eIF2α内质网应激反应途径中发挥重要作用（1-3）。ER应激抑制了大多数mRNA的翻译，但也选择性地刺激了某些基因如ATF-4的翻译（2）。诱导ATF-4表达会增加基因的表达量，这对于从ER应激状态中恢复正常是非常重要的（4）。

1. Fawcett, T.W. et al. (1999) Biochem J 339 ( Pt 1), 135-41.
2. Harding, H.P. et al. (2000) Mol Cell 6, 1099-108.
3. van Huizen, R. et al. (2003) J Biol Chem 278, 15558-64.
4. Yusta, B. et al. (2006) Cell Metab 4, 391-406.

原厂资料：

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-IC=Immunofluorescence (Immunocytochemistry), ChIP=Chromatin IP

Homology

Species predicted to react based on 100% sequence homology: Mouse, Rat

Specificity / Sensitivity

ATF-4 (D4B8) Rabbit mAb recognizes endogenous levels of total ATF-4 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human ATF-4 protein.

Background

ATF-4, an activating transcription factor/cAMP-response element-binding protein family member, functions in the PERK and eIF2α ER stress responsive pathway (1-3). ER stress represses the translation of the majority of mRNAs, but selectively stimulates the translation of certain mRNAs including that of ATF-4 (2). Induced expression of ATF-4 increases the expression of genes critical for the recovery from ER stress (4).

1. Fawcett, T.W. et al. (1999) Biochem J 339 ( Pt 1), 135-41.
2. Harding, H.P. et al. (2000) Mol Cell 6, 1099-108.
3. van Huizen, R. et al. (2003) J Biol Chem 278, 15558-64.
4. Yusta, B. et al. (2006) Cell Metab 4, 391-406.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
*Species cross-reactivity is determined by western blot.
** Anti-rabbit secondary antibodies must be used to detect this antibody.
Recommended Antibody Dilutions:
Western blotting 1:1000
Immunoprecipitation 1:50
Immunofluorescence (IF-IC) 1:200
Chromatin IP 1:100