Micrococcal Nuclease

• 产品编号：CST-10011S      品牌：CST       原厂货号：10011S
• 产品分类：生化试剂 > 化学试剂 > 蛋白酶和磷酸酶抑制剂
• 应用分类：

描述：

Description

Micrococcal nuclease is a relatively non-specific endo-exonuclease derived from Staphylococcus aureus. Active in the pH range of 7.0-10.0, this product digests double-stranded, single-stranded, circular, and linear nucleic acids.

In the SimpleChIP^® Enzymatic Chromatin IP kit assay, following cell lysis, the chromatin is fragmented by partial digestion with micrococcal nuclease to obtain chromatin fragments of 1 to 5 nucleosomes in size. Enzymatic digestion of chromatin is much milder than sonication and eliminates problems due to variability in sonication power and emulsification of chromatin during sonication, which can result in incomplete fragmentation of chromatin or loss of antibody epitopes due to protein denaturation and degradation.

This product is offered to conveniently provide additional micrococcal nuclease when fragmenting chromatin with our SimpleChIP^® (#9002, #9003) and SimpleChIP^® Plus (#9004, #9005) Enzymatic Chromatin IP Kits. These kits provide all the reagents required for performing 6 chromatin preparations (or optimizations) and 30 chromatin immunoprecipitation (ChIP) assays, however there are instances where extra micrococcal nuclease is desired.

原厂资料：

Description

Micrococcal nuclease is a relatively non-specific endo-exonuclease derived from Staphylococcus aureus. Active in the pH range of 7.0-10.0, this product digests double-stranded, single-stranded, circular, and linear nucleic acids.

In the SimpleChIP^® Enzymatic Chromatin IP kit assay, following cell lysis, the chromatin is fragmented by partial digestion with micrococcal nuclease to obtain chromatin fragments of 1 to 5 nucleosomes in size. Enzymatic digestion of chromatin is much milder than sonication and eliminates problems due to variability in sonication power and emulsification of chromatin during sonication, which can result in incomplete fragmentation of chromatin or loss of antibody epitopes due to protein denaturation and degradation.

This product is offered to conveniently provide additional micrococcal nuclease when fragmenting chromatin with our SimpleChIP^® (#9002, #9003) and SimpleChIP^® Plus (#9004, #9005) Enzymatic Chromatin IP Kits. These kits provide all the reagents required for performing 6 chromatin preparations (or optimizations) and 30 chromatin immunoprecipitation (ChIP) assays, however there are instances where extra micrococcal nuclease is desired.

Directions for Use

 Use during chromatin fragmentation in chromatin immunoprecipitation (ChIP) assays as directed in the protocols for the SimpleChIP^® and SimpleChIP^® Plus Enzymatic Chromatin IP Kits.

注意事项：

Storage: Supplied in 10 mM Tris-HCl (pH 7.5), 50 mM NaCl, 1mM EDTA and 50% glycerol. Store at –20°C. Do not aliquot the product. This product is stable for 24 months.
Directions for Use: Use during chromatin fragmentation in chromatin immunoprecipitation (ChIP) assays as directed in the protocols for the SimpleChIP® and SimpleChIP® Plus Enzymatic Chromatin IP Kits.