Phospho-Akt Substrate (RXRXXS*/T*) (23C8D2) Rabbit mAb

• 产品编号：CST-10001S      品牌：CST       原厂货号：10001S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-Akt Substrate (RXRXXS*/T*) (23C8D2) Rabbit mAb兔单抗识别包含-5、-3位是精氨酸的磷酸化丝氨酸/苏氨酸的内源性蛋白质，与周围序列无关。此抗体仅与-3位是精氨酸的磷酸化丝氨酸/苏氨酸的蛋白质有较弱的交叉反应。没有发现其能与相应的非磷酸化的序列或其它基序中的磷酸化丝氨酸/苏氨酸序列发生交叉反应。

该单克隆抗体用Akt底物库肽段免疫动物制备。

精氨酸导向的激酶或AGC家族激酶是很重要的激酶，包括cAMP依赖的蛋白激酶（PKA）、cGMP依赖的蛋白激酶（PKG）、蛋白激酶C、Akt和RSK。这些激酶有相同的底物特异性，-3位上有精氨酸的丝氨酸和苏氨酸（1,2）。Akt在介导细胞内关键反应中扮演重要角色，包括细胞生长和生存、血管生成和转录调节（3-5）。目前已经发现一些Akt底物（如GSK-3、Bad、caspase-9），但还有很多重要底物等待发现。Akt磷酸化保守序列-5、-3位是精氨酸的丝氨酸/苏氨酸（6）。磷酸化Akt底物特异性抗体由Cell Signaling Technology公司研发，用于研究Akt磷酸化调节和精氨酸导向的激酶，也可以用于高通量药物发现。

原厂资料：

Specificity / Sensitivity

Phospho-Akt Substrate (RXRXXS*/T*) (23C8D2) Rabbit mAb recognizes endogenous proteins containing phospho-Ser/Thr preceded by Arg at positions -5 and -3 in a manner largely independent of the surrounding amino acid sequence. Minor cross-reactivity is observed for proteins that contain phospho-Ser/Thr preceded by Arg at position -3 only. No cross-reactivity is observed with the corresponding nonphosphorylated sequences or with other phospho-Ser/Thr-containing motifs.

Source / Purification

Monoclonal antibody is produced by immunizing animals with an Akt substrate peptide library.

Background

An important class of kinases, referred to as Arg-directed kinases or AGC-family kinases, includes cAMP-dependent protein kinase (PKA), cGMP-dependent protein kinase (PKG), protein kinase C, Akt, and RSK. These kinases share a substrate specificity characterized by Arg at position -3 relative to the phosphorylated Ser or Thr (1,2). Akt plays a central role in mediating critical cellular responses including cell growth and survival, angiogenesis, and transcriptional regulation (3-5). While a number of Akt substrates are known (such as GSK-3, Bad, and caspase-9) many important substrates await discovery. Akt phosphorylates substrates only at Ser/Thr in a conserved motif characterized by Arg at positions -5 and -3 (6). Phospho-Akt substrate-specific antibodies from CST are powerful tools for investigating the regulation of phosphorylation by Akt and other Arg-directed kinases, as well as for high throughput kinase drug discovery.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%sodium azide. Store at –20°C. Do not aliquot the antibody.
*Species cross-reactivity is determined by western blot.
** Anti-rabbit secondary antibodies must be used to detect this antibody