pF9A CMV hRluc-neo Flexi? Vector

• 产品编号：C9361      品牌：promega       原厂货号：C9361
• 产品分类：克隆 > 克隆载体和克隆试剂盒 > 载体 > 哺乳动物表达载体
• 应用分类：蛋白质研究 > 蛋白质表达 > 哺乳动物表达 > 哺乳动物表达载体

其它组分：

描述：

这些 Flexi® 载体是通过 CMV 启动，使哺乳动物细胞中带有或不带有选择标签的蛋白进行高水平表达的一类特定载体。 pF9A 载体带有海肾萤光素酶基因，可以作为转染对照。当外源蛋白（如转录因子受体）的表达用于报告基因检测时， pF9A 载体可以与 pGL4 萤火虫萤光素酶的载体配合使用。所有的插入片段可以通过 TNT® T7 无细胞表达快速系统 (Cat.# L1170) 来证实。

原厂资料：

The pF9A CMV hRluc-neo Flexi® Vector is designed for use with the Flexi® System, Entry/Transfer (Cat.#C8640) and the Flexi® System, Transfer (Cat.# C8820). The vector contains a CMV immediate early enhancer/promoter region plus a chimeric intron for mammalian expression, and a T7 promoter for in vitro expression of the protein-coding region. In addition, the vector contains a barnase gene for positive selection of the insert, an ampicillin-resistance gene for selection of the plasmid and unique SgfI and PmeI sites that allow easy insertion and transfer of the insert of interest. The pF9A CMV hRluc-neo Flexi® Vector also contains a unique neomycin-resistance gene fused with a humanized Renilla luciferase (hRluc) gene, under the control of the SV40 promoter, allowing long-term selection of transfected mammalian cells and the ability to monitor those selected cells using a Renilla luciferase assay. Transfection of the pF9A CMV hRluc-neo Flexi® Vector containing a protein-coding region into mammalian cells provides resistance to the antibiotic G-418 (Cat.# V7981 or V8091) and allows selection of transfectants.

注意事项：

Flexi® 载体带有致死性 barnase 基因，可以减少载体构建过程中不带有插入片段的阴性克隆的产生。使用 Flexi® Vector Cloning System ，可将 barnase 基因替换为插入的目的 DNA 片段。在没有插入目的 DNA 片段的情况下，购买的此类载体无法在常用的实验用大肠杆菌菌株中生长。