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OMNIMAX2 CHEMICALLY COMP CELLS

 
包装: 20 RXN
运保温度: Store Competent Cells at -80°C.
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描述:

The One Shot® OmniMAX™ 2 T1R E. coli strain is an improved chemically competent cell line, perfect for use in all cloning applications, including the Gateway® technology. OmniMAX™ 2 T1R cells offer the highest transformation efficiency of any chemically competent cells in a One Shot® format, with >5 x 109 transformants/µg pUC19. They also provide efficient transformation of highly methylated DNA, since OmniMAX™ 2 T1R cells lack the E. coli K12 restriction systems (mcrA Δ(mrr hsdRMS-mcrBC)). In addition, the strain carries the tonA genotype, which confers resistance to T1 and T5 phage infection. This protects your samples and minimizes the possibility of downtime in your lab due to phage contamination. The highly versatile OmniMAX™ 2 T1R strain offers the following benefits:

• Ideal for transformation of Gateway® and TOPO® reactions
• Resistance to T1 and T5 phage (tonA)
• Construction of more representative genomic libraries due to the elimination of mcrA, mcrBC, mrr, and hsdRMS
• Blue/white screening of recombinant clones (lacZΔM15)

Genotype: F´ {proAB lacIq lacZΔM15 Tn10(TetR ) Δ(ccdAB)} mcrA Δ(mrr hsdRMS-mcrBC) Φ 80(lacZ)ΔM15 Δ(lacZYA-argF)U169 endA1 recA1 supE44 thi-1 gyrA96 relA1 tonA panD


注意事项:

For Research Use Only. Not for use in diagnostic procedures.


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