pFN2A (GST) Flexi? Vector

• 产品编号：C8461      品牌：promega       原厂货号：C8461
• 产品分类：克隆 > 克隆载体和克隆试剂盒 > 载体 > 细菌表达载体
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 The pFN2A (GST) Flexi ® Vector (a–c) is designed for use with the Flexi ® System, Entry/Transfer (Cat.# C8640),and the Flexi ® System, Transfer (Cat.# C8820). The vector contains a T7 promoter for bacterial or in vitro protein expression of a protein-coding region. The vector appends an N-terminal glutathione-S-transferase (GST) coding region that can be used to purify the expressed protein. The GST tag contains a TEV protease site for removal of the tag after purification. The vector also contains the lethal barnase gene for positive selection of the insert, an ampicillin-resistance gene for selection of the plasmid and unique SgfI and PmeI sites that allow easy insertion or transfer of the sequence of interest. Inserts containing a protein-coding region can easily be transferred from the pFN2A (GST) Flexi ® Vector to other Flexi ® Vectors with different expression options (Table 1). For more information, see the  Flexi ® Vector Systems Technical Manual #TM254. 

注意事项：

 Flexi® 载体带有致死性 barnase 基因，可以减少载体构建过程中不带有插入片段的阴性克隆的产生。 barnase 基因可被插入的目的 DNA 片段所替代。所购买的这一类载体在没有插入目的 DNA 片段的情况下，带有空载体的常用实验室大肠杆菌菌株不能生长。