Naphthofluorescein has emission maxima of approximately 670 nm in aqueous solution at pH 10. The fluorescence from this dye is pH dependent (pKa ~7.6) requiring a relatively alkaline pH for maximal fluorescence. The carboxylic acid of Naphthofluorescein has emission maxima of approximately 670 nm in aqueous solution at pH 10. The fluorescence from this dye is pH dependent (pKa ~7.6) requiring a relatively alkaline pH for maximal fluorescence. The amine reactive 5-(and-6)-carboxynaphthofluorescein succinimidyl ester can be used to couple this fluorophore to biomolecules of interest.
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For Research Use Only. Not for use in diagnostic procedures.