#BL21 STAR DE3 PLYSS COMBO 20 RXN

产品编号：C602003 品牌：invitrogen 原厂货号：C602003
产品分类：克隆 > 克隆载体和克隆试剂盒 > 细菌菌株和感受态细胞 > 感受态细胞
应用分类：克隆及合成生物学 > 转化 > 化学感受态细胞 > 蛋白质表达用感受态细胞

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描述：

BL21 Star™ E. coli strains are high-performance BL21 hosts designed for improving protein yield in a T7 promoter-based expression system. Because T7 RNA polymerase synthesizes mRNA more rapidly than
E. coli RNA polymerases, transcription from the T7 promoter is uncoupled to translation in E. coli. This results in mRNA transcripts unprotected by ribosomes, which are then subject to enzymatic degradation
by endogenous RNases (1). The reduced level of transcripts in the cell often leads to greatly reduced levels of protein yield (Figure1). The BL21 Star™ strains contain a mutation in the gene encoding
RNaseE (rne131), which is one of the major sources of this mRNA degradation (2). BL21 Star™ cells significantly improve the stability of mRNA transcripts and increase protein expression yield from T7
promoter-based vectors (3) (Figure 2).

BL21 Star™(DE3) is ideal for expressing proteins that are non-toxic to E. coli.

BL21 Star™(DE3)pLysS offers lower 查看更多basal-level expression of heterologous genes than BL21 Star™(DE3). It is designed for expressing proteins that are slightly growth inhibitive to E. coli.

注意事项：

Both BL21 Star™(DE3) and BL21 Star™(DE3)pLysS Chemically Competent E. coli are provided in single-use 50 µl aliquots, with S.O.C. medium, and supercoiled control plasmid. Store competent cells at -80°C