For Research Use Only. Not for use in diagnostic p
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One Shot® TOP10 Chemically Competent E. coli are provided at a transformation efficiency of 1 x 109 cfu/µg plasmid DNA and are ideal for high-efficiency cloning and plasmid propagation. They allow stable replication of high-copy number plasmids and are the same competent cells that come with many of our cloning kits.
Features of One Shot® TOP10 cells include:
• Maximization of cloning efficiency in a single-tube format
• Enhanced genomic DNA cloning capabilities
Easy-to-use One Shot® format
The single-tube, single-use format allows for all steps of the transformation protocol, up to plating, to take place in the same tube, thereby helping to save time and to prevent contamination.
Versatile cloning capabilities
One Shot® TOP10 E. coli cells are similar to the DH10B™ strain, and offer the following features:
• hsdR for efficient transformation of unmethylated DNA from PCR amplifications
• mcrA for efficient transformation of methylated DNA from genomic preparations
• lacZΔM15 for blue/white color screening of recombinant clones
• endA1 for cleaner DNA preparations and better results in downstream applications due to elimination of nonspecific digestion by Endonuclease I
• recA1 for reduced occurrence of nonspecific recombination in cloned DNA