Microspheres (also called latex beads or latex particles) are spherical particles in the colloidal size range that are formed from an amorphous polymer such as polystyrene. Because of the way that the polystyrene chains arrange themselves in the bead, the surface is very hydrophobic in character, making these ideal materials for the adsorption of materials such as proteins.
These carboxylate modified (CML) latex particles are produced by copolymerizing carboxylic acid containing polymers. The result is a latex polymer particle with a highly charged, relatively hydrophilic and somewhat 'fluffy' surface layer. The CML particles are electrosterically stabilized, and are therefore safe in concentrations of electrolyte up to 1M univalent salt. CML modified latex particles are negatively charged with a surface which has a polyelectrolyte character. It is only when the pH is ~10 that all the carboxyl groups are ionized. This is a hydrophilic latex.
Microspheres Are Available in Many Sizes and Surface Functionalities • 25 sizes to choose from—0.02 microns to 16 microns • 20 different surface—for ready conjugation to virtually any material • Surfactant free—ultraclean • Various quantities—from milliliter to 500-liter amounts
Key Applications of Microspheres • Instrument calibration (flow cytometry, microscopy, HTS, HCS) • Flow testing (microfluidics, blood flow, water flow, and air flow) • Cell biology tracers (cell differentiation and cell tracing) • Immunoassays (agglutination tests, ELISA, particle capture, and contrast reagents)
Get More Information Read a technical overview of microspheres and find out about high activity beads, super active beads, specialty beads, and review bead-coupling protocols on the IDC Surfactant-free Latex Beads application page.
We’ll Make a Custom Microsphere Product for You If you don’t see the microsphere you’re looking for in our stocked list, we’ll make a custom microsphere for you. Our custom conjugation service is efficient and confidential, and we guarantee the quality of our work. We are ISO 9001:2000 certified.
For Research Use Only. Not intended for animal or human therapeutic or diagnostic use.
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For Research Use Only. Not for use in diagnostic procedures.
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