The Click-iT® Biotin Glycoprotein Detection Kit provides the second part of a simple and robust technique to identify and characterize glycoproteins by Western blot. In step two, after the incorporation of an azide moiety into protein glycan structures with either a Click-iT® metabolic labeling reagent or the Click-iT® Enzymatic Labeling system, the azide-modified glycoproteins are detected via the chemoselective ligation or click reaction between an azide and an alkyne. With this technique, detection sensitivity is in the low femptomole range and biotin-labeled samples can be detected before or after probing the Western with a primary antibody.
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For Research Use Only. Not for use in diagnostic procedures.