CLICK-IT O-GLCNAC ENZYMATIC LA

• 产品编号：C33368      品牌：invitrogen       原厂货号：C33368
• 产品分类：生化和细胞分析 > 标签、标记试剂盒 > 抗体、蛋白标记试剂盒
• 应用分类：

描述：

The Click-iT® O-GlcNAc Enzymatic Labeling System provides a highly sensitive and efficient method for the in-vitro modification of O-GlcNAc modified proteins. Proteins are enzymatically labeled utilizing the permissive mutant β-1,4-galactosyltransferase (Gal-T1 (Y289L)) which transfers azido-modified galactose (GalNAz) from UDP-GalNAz to O-GlcNAc residues. Then, via the chemoselective ligation or click reaction between an azide and an alkyne, the azido-labeled glycoproteins can then be detected with a Click-iT® Glycoprotein Detection kit for gels (TAMRA or Dapoxyl® alkyne) or Western blots (biotin alkyne). Labeling and detection can be completed in less than 24 hours and the Click-iT® glycoprotein products are compatible with LC-MS/MS and Multiplexed Proteomics™ technologies for in-depth analyses of this important post-translational modification.

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