CLICK-IT GLCNAZ METABOLIC GLYC EACH

• 产品编号：C33367      品牌：invitrogen       原厂货号：C33367
• 产品分类：生化和细胞分析 > 标签、标记试剂盒 > 抗体、蛋白标记试剂盒
• 应用分类：

描述：

The Click-iT® GlcNAz metabolic glycoprotein labeling reagentprovides the first part of a simple and robust two-step technique to identify and characterize intracellular O-GlcNAc glycoproteins. In step one, cultured cells are incubated with the azide-modified glucosamine (GlcNAc). The azido-sugar is incorporated into intracellular O-GlcNAc-containing glycoproteins through the permissive nature of the oligosaccharide biosynthesis pathway. In step two, via the chemoselective ligation or click reaction between an azide and an alkyne, the azido-labeled glycoproteins can then detected with a Click-iT® Glycoprotein Detection kit for gels (TAMRA or Dapoxyl® alkyne) or Western blots (biotin alkyne). The Click-iT® glycoprotein products are compatible with LC-MS⁄MS and Multiplexed Proteomics™ technologies for in-depth analyses of the glycoproteome.

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