CLICK-IT GALNAZ METABOLIC GLYC EACH

产品编号：C33365 品牌：invitrogen 原厂货号：C33365
产品分类：生化和细胞分析 > 标签、标记试剂盒 > 抗体、蛋白标记试剂盒
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描述：

The Click-iT® GalNAz metabolic glycoprotein labeling reagent provides the first part of a simple and robust two-step technique to identify and characterize cell surface O-linked glycoproteins. In step one, cultured cells are incubated with the azide-modified galactosamine (GalNAz). The azido-sugar is metabolically incorporated into cell surface O-linked glycoproteins through the permissive nature of the oligosaccharide biosynthesis pathway. In step two, via the chemoselective ligation or click reaction between an azide and an alkyne, the azido-labeled glycoproteins can then be detected with a Click-iT® Glycoprotein Detection Kit for gels (TAMRA or Dapoxyl® alkyne) or Western blots (biotin alkyne). These Click-iT® products are compatible with LC-MS⁄MS and Multiplexed Proteomics™ technologies for in-depth analyses of the glycoproteome.