TOP10F ONE SHOT 40 RXN

产品编号：C303006 品牌：invitrogen 原厂货号：C303006
产品分类：克隆 > 克隆载体和克隆试剂盒 > 细菌菌株和感受态细胞 > 感受态细胞
应用分类：克隆及合成生物学 > 转化 > 化学感受态细胞

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描述：

One Shot® TOP10F´ Chemically Competent E. coli are identical to TOP10 cells, with the addition of an F´ episome. TOP10 competent cells are provided at a transformation efficiency of 1 x 10⁹ cfu/µg supercoiled DNA and are ideal for high-efficiency cloning and plasmid propagation.

Using One Shot® TOP10F´ Chemically Competent E. coli
The F´ episome carries the tetracycline resistance gene and allows isolation of single-stranded DNA from vectors that have an f1 origin of replication. In addition, the F´ episome carries the lacI^q repressor for inducible expression from trc, tac, and lac promoters using IPTG. TOP10F´ cells require IPTG induction for blue/white color screening.