CellTracker reagents are fluorescent chloromethyl derivatives that freely diffuse through the membranes of live cells. Once inside the cell, these mildly thiol-reactive probes react with intracellular components to produce cells that are both fluorescent and viable for at least 24 hours after loading. CellTracker Orange CMTMR has a low pKa, which ensures that it will exhibit bright, orange fluorescence in the cytoplasm at all physiological pH levels.
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