Calcein AM is a cell-permeant dye that can be used to determine cell viability in most eukaryotic cells. In live cells the nonfluorescent calcein AM is converted to green-fluorescent calcein, after acetoxymethyl ester hydrolysis by intracellular esterases. This dye is also available in our special packaging (C-3100) and resuspended in DMSO (C-3099). For a longer wavelength version of this dye check out our new CellTrace calcein red-orange AM (C-34851).