PCR Nucleotide Mix is a premixed solution containing the sodium salts of dATP, dCTP, dGTP and dTTP, each at a concentration of 10mM in water; the total concentration of nucleotides, therefore, is 40mM (pH 7.5). This solution is ready to use and is optimized for standard polymerase chain reactions and specialty approaches including hot start and reverse transcription PCR (RT-PCR).