收藏本站
热门搜索: A3500  E1910  A1360  25200056  dmem  

CELLLIGHT TUBULIN-RFP 0.1 ML

 
包装:
运保温度: Store at 2 to 6°C
到货周期: 登录后查看
标准价: ¥客户可见
会员价: ¥客户可见
积分:
 
登录之后可加入购物车购买,请您 
对比 收藏

运费与支付说明:

1.含干冰类产品有运费;

2. 必须现金付款或有信用额度的会员才可以直接发货,否则需要等待现金付款信息。

描述:

CellLight® Tubulin-RFP, BacMam 2.0, provides an easy method for the labeling of tubulin with Red Fluorescent Protein (RFP) in live cells. Simply add the reagent to your cells, incubate overnight, and the cells are ready to image in the morning. This ready-to-use construct is transduced/transfected into cells using BacMam 2.0 technology, where it expresses RFP fused to human tubulin. You can observe tubulin-RFP behavior in live cells using a fluorescent imaging system and standard TRITC/RFP filter set. Study dynamic action of tubulin filaments in live cells with almost no cytotoxicity. In addition, you can co-transduce/transfect more than one CellLight® reagent and label live cells with multiple tracking or tracing dyes to image dynamic cellular processes. Cells expressing CellLight® constructs can also be fixed with formaldehyde for multiplexing with antibodies using immunocytochemical techniques.

CellLight® Technology
• Highly efficient: up to 90% transduction of a wide range of mammalian cell lines, including primary cells, stem cells, and neurons
• Fast and convenient: simply add CellLight® reagent to your cells, incubate overnight, and image—or store frozen, assay-ready cells for later use
• Less toxic: CellLight® reagents are non-replicating in mammalian cells and are suitable for biosafety level (BSL) 1 handling
• Flexible: co-transduce more than one BacMam reagent for multiplex experiments or co-localization studies; tightly control expression levels by simply varying the dose

Learn more about CellLight® fluorescent protein labeling

BacMam Technology
CellLight® Tubulin-RFP, BacMam 2.0, is a fusion construct of human tubulin and TagRFP, providing accurate and specific targeting to cellular tubulin-RFP. This fusion construct is packaged in the insect virus baculovirus, which does not replicate in human cells and is designated as safe to use with biosafety level (BSL) 1 in most laboratories. BacMam technology ensures that most mammalian cell types are transduced/transfected with high efficiency and minimal toxicity. This transient transfection can be detected after overnight incubation for up to five days—enough time to carry out most dynamic cellular analyses. Like any transfection/transduction technique, the BacMam method does not transfect/transduce all of the cells with equal efficiency, making it poorly suited to cellular population studies or automated imaging/counting. CellLight® reagents are ideal for experiments where cellular or subcellular co-locatization is required, or for cellular function studies that need special resolution.


注意事项:

For Research Use Only. Not for use in diagnostic procedures.
 


相关图片

本产品可用于的实验

最近浏览产品

京ICP备15036693号-2    京公网安备11010802025653    版权所有:北京逸优科技有限公司       0.04

 纠 错