K. lactis YCT 284 Competent cells

产品编号：C1002S 品牌：New England Biolabs 原厂货号：C1002S
产品分类：克隆 > 克隆载体和克隆试剂盒 > 细菌菌株和感受态细胞 > 感受态细胞
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包装：

5 rxns

运保温度:

-80°C

到货周期：

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描述：

Description:
Chemically competent Kluyveromyces lactis YCT284 (∆cts1) cells are a preferred host for secreting heterologous proteins carrying a chitin binding domain tag. YCT284 cells can be transformed with any linearized pKLAC-series expression vector. K. lactis strain YCT284 carries a selectable marker-free deletion of the KLLA0C04730g locus encoding an extracellular chitinase (KlCts1p) which can degrade chitin chromatography resin and compete for resin binding sites (1).

Strain: K. lactis strain YCT284 (∆cts1). No auxotrophies or genetic markers.

Reagents Supplied with Cells: NEB Yeast Transformation Reagent (NEB #M2570). Store at 4°C.

注意事项：

General notes:Store Competent Cells at -80°C. Once Thawed, Do Not Re-freeze.

Usage notes:

Due to the high transformation efficiency of K. lactis YCT284 (∆cts1) Competent Cells, plating multiple dilutions of the cell mixture is necessary to ensure formation of plates with distinct single colonies. Growth time should not exceed 5 days as small colonies that lack an integrated expression fragment may form.

Plates containing colonies can be stored at 4°C for up to two weeks.

K. lactis YCT284 (∆cts1) Competent Cells may form small clumps when grown in liquid culture (1). This can obscure cell density measurements using light scattering techniques.

The deletion of locus KLLA0C04730g in YCT284 can be confirmed by PCR using the primers GGTCACCAGAAATACAAG and ATAAAAATATGATAAGGCTACACG to amplify a 2.0 kb diagnostic fragment.

The chitin binding domain (KlChBD) derived from the K. lactis secreted Cts1p chitinase (1) is not suitable for cytoplasmic expression and purification of KIChBD-tagged proteins.