Figure 1. miRNA mimic function was assayed in HeLa cells 24 and 48 h after transfection of 10 nM miR-375 native-based mimic, modified native-based mimic, or miRIDIAN microRNA Mimic using a dual luciferase reporter system and normalized to the control (no mimic).
Figure 2. miRIDIAN microRNA Mimic function for 11 human miRNAs was assayed in HeLa and HepG2 cells 48 h after transfection of 10 nM mimic using a dual luciferase reporter system and normalized to the control (no mimic). Similar results were obtained with 1 nM miRIDIAN microRNA Mimic.
产品用途：
通过meRIDIAN microRNA加强miRNA的活性从而研究功能获得性效应
检测miRNAs所调节的基因表达和评价对信号通路影响效应；
阐明miRNA在诸如细胞发育、增殖、分化和凋亡中的机理；
鉴定和确认miRNA靶标。
原厂资料：
Thermo Scientific miRIDIAN microRNA Mimics are double-stranded RNA oligonucleotides designed to mimic the function of endogenous, mature microRNAs. miRIDIAN Mimics are chemically enhanced with the ON-TARGET modification pattern to preferrentially program RISC with the active microRNA strand. Pre-designed mimics are available for all human, mouse, and rat microRNAs in the miRBase Sequence Database 16.0.
Highlights
Superior performance in comparison to native, double-stranded microRNA (see Figure 1, Supporting Data)
Highly effective mimic of endogenous mature microRNA function (see Figure 2, Supporting Data)
Preferential programming of RISC with active strand of microRNA
Exclusion of passenger strand through proprietary chemical modification patterning (ON-TARGET)
Applications
Supplement microRNA activity to study gain-of-function effects
Screen for microRNAs that regulate gene expression and affect cellular pathways
Elucidate microRNA involvement in normal biological and disease pathways
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