Chemically defined cryo-preservation medium. Does not contain FBS (foetal bovine serum) or BSA (bovine serum albumin).
Intended Use
TC-Protector is a ready-to-use optimised cryo-preservation medium which allows for the direct freezing of an expansive range of mammalian cell lines at -80oC.
TC-Protector provides a no-mess solution for the protection of cells during frozen storage, and reliably gives a high cell viability and durability rate on thawing.
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
Tissue Culture
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Storage
Store unused product at +4oC.
Shelf Life
24 months from date of despatch.
Instructions For Use
1. Centrifuge cells pre-suspended in complete growth medium at 1,000-1,500rpm for 60 seconds.
2. Remove supernatant and resuspend cells in TC-Protector at a concentration of 5x106to 1x107cells/mL.
3. Transfer this cell suspension to cryogenic storage vial.
4. Freeze vial at -80oC (liquid nitrogen should be used for long-term storage).
Health And Safety Information
This product contains 10% dimethyl sulfoxide (DMSO): a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
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