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TMB PLUS

 
包装: 500 ml
运保温度: +4oC
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描述:

Product Form

Ready to use TMB solution - liquid

Intended Use

BUF042B is a high performance TMB (3,3´, 5, 5´- tetramethylbenzidine) solution, recommended for use in ELISA as a substrate for horseradish peroxidase (HRP).

BUF042B contains TMB, substrate buffer and hydrogen peroxide in a safe, ready to use solution. The activity of TMB has been optimised to enable increased sensitivity, minimal background and rapid development.

BUF042B produces a deep blue colour during the enzymatic degradation of H2O2 by Horseradish Peroxidase. The reaction may be stopped with 0.2M sulphuric acid, resulting in a yellow colour read at 450nm.

Application

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name Yes No Not Determined Suggested Dilution
ELISA       Neat
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Storage

Store at +4oC. DO NOT FREEZE.
This product is photosensitive and should be protected from light.

Avoid exposure to heat and contamination with metal ions or peroxidase.

Store in bottles made of High Density Polyethylene (HDPE).

Shelf Life

18 months from date of despatch.

Instructions For Use

1. It is recommended that 100ul of BUF042B TMB substrate is used per microtiter well. Pour the desired amount of substrate into a sealed container and allow it to reach room termperature in the dark.

2. Add 100ul substrate solution per microtiter well.

3. Allow development of the substrate solution. Time of development is typically 5-30 minutes. For best results, the plate should be kept in the dark during incubation e.g. wrapped in tinfoil.

4. For kinetic assays, read absorbance at 655nm (blue). For endpoint assays, add an equal volume of 0.2M sulphuric acid and read the absorbance at 450nm (yellow). This endpoint solution is stable for up to one hour.

N.B. If reduced intensity is required, it is recommended that the development time is reduced or the antibody/conjugate is diluted further. (Dilution of BUF042B is not recommended).

Health And Safety Information

Material Safety Datasheet Documentation #10100 available at: 
http://www.abdserotec.com/uploads/MSDS/10100.pdf

Regulatory

For research purposes only

 

 

 


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