BUF037B is an ELISA assay diluent designed to reduce background noise and assist in matrix equalization in problematic serum, plasma or cell culture samples. Designed for use in all sandwich ELISA formats, it contains mammalian serum proteins to reduce non-specific interactions between sample proteins and the plate surface.
The difference in complexity, between serum or plasma samples and diluted standard curve proteins, is a potential cause of target analyte under-recovery. BUF037B equalizes the matrix, inhibits complement and thrombin activity and contains an antimicrobial agent.
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
Neat
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Storage
Store at +4oC.
DO NOT FREEZE
Shelf Life
18 months from date of despatch.
Instructions For Use
Pipette buffer directly onto the plate, just prior to adding the sample and standard (suggested volume 50 - 100 ul/well).
NB. Since this buffer contains proteins derived from goat, BUF037B is not recommended for ELISA where an anti goat secondary antibody is used.
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