BUF034B is a novel protein-free blocking buffer suitable for use in all ELISA formats requiring maximum blocking strength. With Tween and synthetic blocking agents, the inert nature of this unique buffer, enables maximum reduction of non-specific binding and interference associated particularly with sandwich ELISA assays.
Additional molecular stabilizers and an antimicrobial agent provide a long-term stable environment for coating antigen or capture antibody. Plates can be blocked at room temperature and stored once dried for up to a year at +4°C.
N.B. SYNBLOCK is not suitable for use on Immunolon-2 plates. AbD Serotec recommends the use ofBUF033Afor this purpose.
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
Neat
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Storage
Store at +4oC.
DO NOT FREEZE
Shelf Life
18 months from date of despatch.
Instructions For Use
1. Coat ELISA plate with antibody or antigen as required.
2. After incubation, remove the coating solution and wash the plate x2 with wash buffer.BUF031Acan be used for this purpose.
3. Add 300-400ul of BUF034B and incubate for 2-24 hours. Use a volume equal to or greater than the volume of coating solution.
4. After removal of the blocking buffer continue with the assay or dry the plate for long-term storage at +4°C.
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