AEC chromogen concentrate is intended for use with AEC substrate bufferBUF020for use in immunohistochemistry procedures. It produces a reddish/brown precipitate localised to the site of antigen detected by immunohistochemical staining utilising horseradish peroxidase enzyme labels.
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
Immunohistology - Frozen
Immunohistology - Paraffin
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Storage
Store at +4oC. DO NOT FREEZE. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of despatch.
Instructions For Use
1. Dilute AEC Chromogen concentrate 1:50 with AEC Substrate BufferBUF020.
2. Apply mixture to tissue section.
3. Incubate the slides for 5 - 10 min at room temperature.
4. Rinse tissue sections with deionized water.
5. Counterstain and mount using aqueous mounting media.
6. AEC induces a red precipitate with almost no background.
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