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alamarBlue®

 
包装: 100 ml
运保温度: RT
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描述:

Product Information

alamarBlue® is designed to provide a rapid and sensitive measure of cell proliferation and cytotoxicity in various human and animal cell lines, bacteria and fungi. 

alamarBlue® is an indicator dye, that incorporates an oxidation-reduction (REDOX) indicator that both fluoresces and changes colour in response to the chemical reduction of growth medium, resulting from cell growth. The alamarBlue® assay is designed to quantitatively measure the proliferation of various human and animal cell lines, bacteria and fungi.

Full instructions can be found at www.abdserotec.com/uploads/IFU/BUF012A.pdf

For further information and Technical help, please visit www.abdserotec.com/alamarBlue
This site includes: 
Frequently Asked Questions
Example calculations
Product-related references

Intended Use

• The bioassay can be used to establish proliferation or relative cytotoxicity. 
• Baseline data for predicting the toxicity of related novel agents can be compared to baseline data with known in-vivo toxicity.
• alamarBlue® is for use between pH6.8 and pH7.4.
 

Test Principle

• Growing cells cause a chemical reduction of alamarBlue®.
• Continued growth maintains a reduced environment. (fluorescent, red).
• Inhibition of growth maintains an oxidized environment. (non-fluorescent, blue).
• Data may be collected using either fluorescence-based or absorbance-based instrumentation. 
• Fluorescence is monitored at 530-560nm excitation wavelength and 590nm emission wavelength. 
• Absorbance is monitored at 570nm and 600nm.

 

Application

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.

 

Application Name Yes No Not Determined Suggested Dilution
ELISA        
Functional Assays        
Immunofluorescence        
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

 

Storage

This product may be stored for 12 months at room temperature.
Store at 2-8oC to increase the shelf life to 20 months.

This product should be stored undiluted.
This product is photosensitive and should be protected from light. 
 

Shelf Life

12 months from date of despatch.

Instructions For Use

Instructions for use can be found at www.abdserotec.com/uploads/IFU/BUF012B.pdf

Health And Safety Information

Material Safety Datasheet Documentation #10289 available at: 
http://www.abdserotec.com/uploads/MSDS/10289.pdf

Acknowledgements

Manufactured for AbD Serotec by Trek Diagnostic System. U.S. patent 5,501,959.

Regulatory

For research purposes only

 


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