alamarBlue®is designed to provide a rapid and sensitive measure of cell proliferation and cytotoxicity in various human and animal cell lines, bacteria and fungi.
alamarBlue®is an indicator dye, that incorporates an oxidation-reduction (REDOX) indicator that both fluoresces and changes colour in response to the chemical reduction of growth medium, resulting from cell growth. The alamarBlue®assay is designed to quantitatively measure the proliferation of various human and animal cell lines, bacteria and fungi.
For further information and Technical help, please visitwww.abdserotec.com/alamarBlue
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Frequently Asked Questions
Example calculations
Product-related references
Intended Use
• The bioassay can be used to establish proliferation or relative cytotoxicity.
• Baseline data for predicting the toxicity of related novel agents can be compared to baseline data with known in-vivo toxicity.
• alamarBlue®is for use between pH6.8 and pH7.4.
Test Principle
• Growing cells cause a chemical reduction of alamarBlue®.
• Continued growth maintains a reduced environment. (fluorescent, red).
• Inhibition of growth maintains an oxidized environment. (non-fluorescent, blue).
• Data may be collected using either fluorescence-based or absorbance-based instrumentation.
• Fluorescence is monitored at 530-560nm excitation wavelength and 590nm emission wavelength.
• Absorbance is monitored at 570nm and 600nm.
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
Functional Assays
Immunofluorescence
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Storage
This product may be stored for 12 months at room temperature.
Store at 2-8oC to increase the shelf life to 20 months.
This product should be stored undiluted.
This product is photosensitive and should be protected from light.
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