Description: This FlowCytomix Simplex Kit is designed for the measurement of Human sICAM-1 in an immunoassay analyzed on a flow cytometer. Together with the FlowCytomix Human Basic Kit PE (cat. BMS8421FF), this kit can be used to detect sICAM-1 alone or can be multiplexed with other Simplex Kits to measure a variety of analytes.
This kit contains bead population A11.
Intercellular Adhesion Molecule-1 (ICAM-1) is a member of the immunoglobulin supergene family and functions as a ligand for the Lymphocyte Function-Associated Antigen-1 (LFA-1), an alpha-beta-complex that is a member of the leukocyte integrin family of cell-cell and cell-matrix receptors. This family consists of the leukocyte adhesion glycoproteins LFA-1 which mediates lymphocyte adhesion, Mac-1 which mediates granulocyte adhesion and p150,95.
ICAM-1 is a single-chain glycoprotein with a polypeptide core that can be expressed on non-hematopoietic cells of many lineages such as vascular endothelial cells, thymic epithelial cells, other epithelial cells and fibroblasts and on hematopoietic cells such as tissue macrophages, mitogen-stimulated T-lymphoblasts, germinal center B-cells and dendritic cells in tonsils, lymph nodes and Peyer's patches. ICAM-1 is inducible on fibroblasts and endothelial cells by inflammatory mediators such as IL-1, TNF and IFN-gamma within few hours and is correlated to the infiltration of lymphocytes into inflammatory lesions. ICAM-1 seems to be the initial marker of inflammatory reactions and is expressed prior to, and to a greater extent than is HLA-DR.
The role of ICAM-1 as a disease marker has been demonstrated for a number of different indications and pathological situations.
Components
1 vial (175 ul) Fluorescent Beads (20x) coated with monoclonal antibody to human sICAM-1, Bead Population A11
2 vials human sICAM-1 Standard (lyophilized): 80 ug/ml upon reconstitution
1 vial (350 ul) PE-Conjugate (20x) anti-human sICAM-1 monoclonal antibody
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