Description: The monkey sTNF-R I ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of monkey sTNF-R I. The monkey sTNF-R I ELISA is for research use only. Not for diagnostic or therapeutic procedures.
Tumor Necrosis Factor (TNF) was originally discovered in sera of animals and was found to cause hemorrhagic necrosis of some transplantable mouse and human tumors and to exhibit primarily cytotoxic activities against tumor but not normal cells in vitro. The TNF family consists of two proteins designated TNF-alpha, also called cachectin, and TNF-beta, also called lymphotoxin, which are pleiotropic cytokines that can mediate a wide variety of biological effects.
Both TNF-alpha and TNF-beta have been shown to interact with a cell through specific high affinity receptors with a few hundred up to more than 20,000 copies per cell. TNF-receptors have been demonstrated on a wide variety of somatic cells including fibroblasts, endothelial cells, adipocytes, liver membranes, granulocytes and several tumor cell lines.
Components
Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to monkey sTNF-R I
HRP-Conjugate anti-monkey sTNF-R I monoclonal antibody
Monkey sTNF-R I Standard concentrate, 12 U/ml upon dilution
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Blue-Dye
Green-Dye
Adhesive Films
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