Description: The monkey sTNF-R I Instant ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of soluble monkey Tumor Necrosis Factor-Receptor I. The monkey sTNF-R I Instant ELISA is for research use only. Not for diagnostic or therapeutic procedures.
Tumor Necrosis Factor (TNF) was originally discovered in sera of animals and was found to cause hemorrhagic necrosis of some transplantable mouse and human tumors and to exhibit primarily cytotoxic activities against tumor but not normal cells in vitro. The TNF family consists of two proteins designated TNF-alpha, also called cachectin, and TNF-beta, also called lymphotoxin, which are pleiotropic cytokines that can mediate a wide variety of biological effects.
Both TNF-alpha and TNF-beta have been shown to interact with a cell through specific high affinity receptors with a few hundred up to more than 20,000 copies per cell. TNF-receptors have been demonstrated on a wide variety of somatic cells including fibroblasts, endothelial cells, adipocytes, liver membranes, granulocytes and several tumor cell lines.
Components
Aluminium pouch(es) with a Microwell Plate coated with monoclonal Antibody (murine) to monkey sTNF-R I, HRP-Conjugate (anti-monkey sTNF-R I monoclonal (murine) antibody) and Assay Buffer, lyophilized
Aluminium pouch(es) with a monkey sTNF-R I Standard curve (coloured)
Wash Buffer Concentrate 20x (phosphate-buffered saline with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Assay Buffer Concentrate 20x (Use when an external predilution of the samples is needed)
Stop Solution (1M Phosphoric acid)
Adhesive Plate Covers
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