Description: The mouse IL-12 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of mouse IL-12. The mouse IL-12 ELISA is for research use only. Not for diagnostic or therapeutic procedures.
Interleukin-12 is produced primarily by stimulated macrophages. IL-12 has been shown to be a proinflammatory cytokine produced by phagocytic cells, B cells, and other antigen - presenting cells that modulate adaptive immune responses by favoring the generation of T-helper type 1 cells.
IL-12 exerts a variety of biological effects on T and natural killer cells. Apart from promotion of Th1 development and its ability to promote cytolytic activity it mediates some of its physiological activities by acting as a potent inducer of IFN-gamma production and the stimulation of other cytokines from peripheral blood T and NK cells. IFN-gamma then enhances the ability of the phagocytic cells to produce IL-12 and other proinflammatory cytokines. Thus, IL-12 induced IFN- gamma acts in a positive feedback loop that represents an important amplifying mechanism in the inflammatory response to infections.
Its role in directing development of a Th1 type immune response from naive T cells demonstrates its critical role in regulation of the immune response and strongly suggests its potential usefulness in cancer therapy.
Components
Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to mouse IL-12
Biotin-Conjugate anti-mouse IL-12 monoclonal antibody
Streptavidin-HRP
Mouse IL-12 Standard lyophilized, 4 ng/ml upon reconstitution
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Blue-Dye
Green-Dye
Red-Dye
Adhesive Films
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