Description: The mouse IL-5 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of mouse IL-5. The mouse IL-5 ELISA is for research use only. Not for diagnostic or therapeutic procedures.
Mouse IL-5 has been described as T-cell replacing factor and as B-cell growth factor II based on its induction of proliferation and Ig secretion by activated B cells. Several other activities have furthermore been attributed to this cytokine, including induction of growth and differentiation of eosinophils from bone marrow stew cells, induction of mIL-2R expression on both normal splenic and activated B cells and enhancements of IgA secretion by LPS-stimulated B-cells. mIL-5 has been shown to be produced exclusively by the TH2 subset of mouse TH clones. The spectrum of biological activities of mIL-5, on B cells as well as other cell types suggests that mIL-5 plays an important functional role in the activities of TH2 cells. mIL-5 is a heavily glycosylated glycoprotein.
Components
Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to mouse IL-5
Biotin-Conjugate anti-mouse IL-5 monoclonal antibody
Streptavidin-HRP
Mouse IL-5 Standard lyophilized, 1000 pg/ml upon reconstitution
Sample Diluent
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Blue-Dye
Green-Dye
Red-Dye
Adhesive Films