Description: The mouse IFN-alpha ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of mouse IFN-alpha. The mouse IFN- alpha ELISA is for research use only. Not for diagnostic or therapeutic procedures.
The interferons represent proteins with antiviral activity secreted from cells in response to a variety of stimuli. The interferons exhibit a huge number of biological effects that play major roles in both innate and adaptive immunity. The antiproliferative and antitumor activities of interferon have led to the application as an antitumor agent. Additionally, the protein has been shown to sensitize lymphocytes to undergo apoptosis and enhance the proliferation and differentiation of CD8 T-cells. Alterations in surface antigens may be an important mechanism by which interferon can modulate cellular interactions.
In mammals, class I interferon (IFN) genes form a superfamily consisting of three gene families, the alpha interferon (IFN-alpha), the beta interferon (IFN-beta) and the interferon omega (IFN-omega) genes. Mouse and human genomes carry more than a dozen genes coding for closely related alpha interferon subtypes. Most IFN subtypes and limitin, are thought to bind the same receptor, raising the question of whether different IFN subtypes possess specific functions. There are 14 IFN-alpha subtypes in the mouse which share at least 75% identity in protein sequence. The genes coding for all known interferons alpha/beta have been located to murine chromosome 4.The mouse IFN-a ELISA Kit recognises only the expression of IFN-a2 and IFN-a4 subtypes.
Components
Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to mouse IFN-alpha
Biotin-Conjugate anti-mouse IFN-alpha monoclonal antibody
Streptavidin-HRP
Mouse IFN-alpha Standard lyophilized, 4000 pg/ml upon reconstitution
Calibrator Diluent
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Blue-Dye
Green-Dye
Red-Dye
Adhesive Films