Description: The mouse IL-33 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of mouse IL-33. The mouse IL-33 ELISA is for research use only. Not for diagnostic or therapeutic procedures.
Mouse IL-33 is a 30-kDa member of the IL-1 family produced by endothelial and epithelial cells. IL-33 plays a pro-inflammatory role in the immune system by inducing IL-5 and IL-13 and by activating basophils, eosinophils and mast cells. In addition, IL-33 decreases inflammation by interacting with IL-1 and blocking its effect. Expression of IL-33 can be upregulated by IL-1beta. In addition to gastrointestinal and lung tissue, IL-33 is expressed in the central nervous system, specifically astrocytes. This cytokine is localized primarily in the nucleus where it is thought to play a role in transcriptional repression. Upon wounding or infection, IL-33 is released from the cell via an atypical secretory pathway. Necrosis-mediated release of IL-33 enables binding to ST2, a member of the IL-1 receptor family found on mast and Th2 effector cells, leading to the release of Th2 cytokines. During apoptosis, IL-33 is cleaved by caspases-3 and 7 (and most likely not caspase-1) to produce an 18-kDa fragment which is released. This cleaved form of IL-33 can bind the ST2 receptor, but no signaling events are initiated. This dual pattern of expression (nuclear and extracellular) is reminiscent of alarmins also known as endogeneous danger signals. Other known alarmins are IL-1a and HMGB1.
Components
Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to mouse IL-33
Biotin-Conjugate anti-mouse IL-33 monoclonal antibody
Streptavidin-HRP
Mouse IL-33 Standard lyophilized, 6 ng/ml upon reconstitution
Sample Diluent
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Blue-Dye
Green-Dye
Red-Dye
Adhesive Films
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