Description: The mouse IL-27 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of mouse IL-27. The mouse IL-27 ELISA is for research use only. Not for diagnostic or therapeutic procedures.
IL-27, a member of the IL-12 family, is a heterodimeric protein consisting of the p40-related protein Epstein-Barr virus-induced gene 3 (EBI3) noncovalently linked to an IL-12p35-related protein, p28 (also known as IL-30). IL-27 is produced by activated antigen presenting cells and mature dendritic cells. LPS-stimulated IL-27 production is dependent on the TLR4/MyD88 mediated pathway. IL-27 prevents the development of Th17 cells even in the presence of TGF-beta and IL-6, but it has little or no effect on committed Th17 cells. Recent studies indicate that IL-27 has potent anti-tumor activity. In vitro, IL-27 has been found to act directly on naive CD8 cells, generating CTL with enhanced granzyme B expression. In vivo, IL-27 has been reported to augment CTL activity, inhibit tumor growth, and induce complete regression of primary and metastatic neuroblastoma tumors. It has been reported that IL-27 is critically involved in the induction of inflammation in rheumatoid arthritis.
Components
Aluminium pouch(es) with a Microwell Plate coated with polyclonal antibody to mouse IL-27
Biotin-Conjugate anti-mouse IL-27 polyclonal antibody
Streptavidin-HRP
Mouse IL-27 Standard lyophilized, 2000 pg/ml upon reconstitution
Sample Diluent
Calibrator Diluent
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Blue-Dye
Green-Dye
Red-Dye
Adhesive Films