Description: The mouse sCD40L ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of mouse sCD40L. The mouse sCD40L ELISA is for research use only. Not for diagnostic or therapeutic procedures.
The murine cell surface antigen is known also as Ly62. CD40 belongs to the TNF receptor superfamily and is a biochemical marker for the identification of activated platelets of patients with cardiovascular diseases (angina pectoris, infarction, stroke etc.)
A key role of CD40/CD40ligand interactions in immune activation, particularly in T-cell dependent B cell responses is anticipated. CD40 Ligand is expressed on the surface of activated CD4+ T cells, basophils, and mast cells. Binding of CD40L to its receptor, CD40, on the surface of B cells stimulates B-cell proliferation, adhesion and differentiation. A soluble isoform of CD40L has been shown to exist in the circulation.
Components
Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to mouse sCD40L
Biotin-Conjugate anti-mouse sCD40L monoclonal antibody
Streptavidin-HRP
Mouse sCD40L Standard lyophilized, 40 ng/ml upon reconstitution
Sample Diluent
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Blue-Dye
Green-Dye
Red-Dye
Adhesive Films
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