Description: The human sVEGF-R1 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human sVEGF-R1. The human SVEGF-R1 ELISA is for research use only. Not for diagnostic or therapeutic procedures.
Soluble VEGF-R1 (sFLT-1) is a naturally occurring endogenous form of the VEGF-R1 and was originally found in the supernatant of human vascular endothelial cells. It is generated by differential splicing of the flt-1 gene. In vitro sVEGF-R1 is used to inhibit VEGF-A mediated signals in endothelial cells and in vivo it can be used to block physiological angiogenesis in several organs, e.g. in the ovary or in bones. Tumor cells transfected with the flt-1 gene are growth restricted in vivo because of the limitation in developing tumor blood vessels via VEGF-A signalling.
Characterization of angiogenic activity, such as embryonic development, placental vascularization, cancer and wound healing is measured by comparing the ratio of angiogenic stimulators (e.g. FGF-2, FGF-1, VEGF-A, Ang-1) to angiogenic inhibitors (e.g. sFLT-1, angiostatin, endostatin, thrombospondin).
Components
Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human sVEGF-R1
Biotin-Conjugate anti-human sVEGF-R1 polyclonal antibody
Streptavidin-HRP
Human sVEGF-R1 Standard lyophilized, 20 ng/mL upon reconstitution
Conjugate Diluent
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Blue-Dye
Green-Dye
Red-Dye
Adhesive Films
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