Description: The human sCD40 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human sCD40. The human sCD40 ELISA is for research use only. Not for diagnostic or therapeutic procedures.
CD40 is a 48 kD type I glycoprotein and a member of the TNFR superfamily primarily expressed on B cells, macrophages, follicular dendritic cells, endothelial cells, fibroblasts, and at low levels on plasma cells. CD40 is also expressed on a wide range of tumor cells, including non-Hodgkin's and Hodgkin's lymphomas, myeloma and some carcinomas including nasopharynx, bladder, cervix, kidney and ovary.The binding of CD154 (CD40L) on TH cells to CD40 activates antigen presenting cells and induces a variety of downstream effects.
Components
Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human sCD40
Biotin-Conjugate anti-human sCD40 monoclonal antibody
Streptavidin-HRP
Human sCD40 Standard lyophilized, 1 ng/ml upon reconstitution
Sample Diluent
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Blue-Dye
Green-Dye
Red-Dye
Adhesive Films
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