Description: The human p53 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human p53. The human p53 ELISA is for research use only. Not for diagnostic or therapeutic procedures.
p53 is a tumor suppressor that is regulated by transcriptional, post-transcriptional, and post-translational mechanisms in response to stress. Studies demonstrate that p53 is critical for the prevention of tumorigenesis and may potentially be involved in at least half of all known cancers. p53 functions as a transcription factor to regulate genes involved in apoptosis, cell cycle, senescence, metabolism, angiogenesis, immunity, differentiation, and migration. Moreover, p53 is phosphorylated at multiple sites, including Ser15, following DNA damage.
Components
Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human p53
Biotin-Conjugate anti-human p53 monoclonal antibody
Streptavidin-HRP
Human p53 Standard lyophilized, 100 U/ml upon reconstitution
Sample Diluent
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Blue-Dye
Green-Dye
Red-Dye
Adhesive Films
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