Description: The human sAPO-1/Fas ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human sAPO-1/Fas. The human sAPO-1/Fas ELISA is for research use only. Not for diagnostic or therapeutic procedures.
APO-1/Fas (CD95), a member of the TNF/NGF receptor superfamily, is a glycosylated 48 kDa surface protein containing a single transmembrane region. APO-1 is expressed on a variety of human B and T cell lines, on many different tumor cells and on various normal human tissues. APO-1 also occurs in a soluble form (sAPO-1) devoid of a transmembrane region. Triggering of APO-1 by its ligand or by certain anti-APO- 1 monoclonal antibodies results in rapid induction of programmed cell death, apoptosis, in susceptible cells. The tissue distribution of APO-1 and of the APO-1 ligand suggests that the APO-1 receptor/ligand system plays an important role in various aspects of mammalian development and especially in the homeostasis of the immune system.
Expression of the APO-1 cell surface protein is enhanced by IFN-gamma and TNF and by activation in lymphocytes.
Components
Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human sAPO-1/Fas
Biotin-Conjugate anti-human sAPO-1/Fas monoclonal antibody
Streptavidin-HRP
Human sAPO-1/Fas Standard lyophilized, 2 ng/ml upon reconstitution
Sample Diluent
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Green-Dye
Red-Dye
Adhesive Films
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