Description: The human Bcl-2 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human Bcl-2. The human Bcl-2 ELISA is for research use only. Not for diagnostic or therapeutic procedures.
B-cell lymphoma 2 encoded by a proto-oncogene, is an intracellular membrane-associated protein that functions to block programmed cell death. Expression of Bcl-2 has been reported in a wide range of hematopoietic cells, nonneoplastic epithelia and epithelial malignancies. Bcl-2 levels are low or undetectable in endothelial cells. A decrease in Bcl-2 levels leads to cell death by apoptosis.
Overexpression of Bcl-2 on the other hand protects cells from death, but it is neither able to immortalize normal cells, nor to cause tumorgenic transformation of immortalized cells.
Components
Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human Bcl-2
Biotin-Conjugate anti-human Bcl-2 monoclonal antibody
Streptavidin-HRP
Human Bcl-2 Standard lyophilized, 64 ng/mL upon reconstitution
Sample Diluent
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Lysis Buffer 10x
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Blue-Dye
Green-Dye
Red-Dye
Adhesive Films
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