Description: The human LIF ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human LIF. The human LIF ELISA is for research use only. Not for diagnostic or therapeutic procedures.
Leukemia inhibitory factor (LIF) is a cytokine which was primarily described as a product of activated T lymphocytes and macrophages. It is a variably glycosylated 180 amino acid protein of apparent molecular weight between 35 and 67 kDa depending on its source and glycosylation. The human LIF-gene is located on chromosome 22, the gene product is highly conserved between species. The multivarious nature of LIF is illustrated by its multiple "discoveries" under a variety of synonyms.
LIF exerts its pleiotropic functions in a wide variety of systems. Examples for its important and diverse physiological functions are support of growth and inhibition of differentiation of normal embryonic stem cells, stimulation of the production of acute-phase proteins by hepatocytes.
A soluble form of LIF can be detected in human body fluids such as serum, whole blood, synovial fluid and urine.
Components
Aluminium pouch(es) with a Microwell Plate coated with monoclonalantibody to human LIF
Biotin-Conjugate anti-human LIF monoclonal antibody
Streptavidin-HRP
Human LIF Standard, 400 pg/mL
Sample Diluent
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Blue-Dye
Green-Dye
Red-Dye
Adhesive Films
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