Description: The human sCD30 Instant ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human soluble CD30 (Ki-1). The human sCD30 Instant ELISA is for research use only. Not for diagnostic or therapeutic procedures.
Characterization of the CD30 antigen has shown it to be in its mature form a transmembrane protein of about 120kDa elaborated from an 84kD cytoplasmic precursor primarily through glycosylation. The CD30 ligand (CD30L) has been identified, showing significant homology to TNF-alpha, TNF-beta, FasL, CD40L, CD27L and 4-1BBl. CD30L is expressed on activated T-cells. Interactions of the cytokine receptor CD30 with its ligand induces pleiotropic biologic effects, such as differentiation, activation, proliferation and cell death.
CD30 expression is strictly dependent on activation and proliferation of T- and B-cells. In pathological conditions, CD30 positivity is regarded as a peculiar attribute of Hodgkin's and Reed-Sternberg cells.
An 85kDa soluble form of the CD30 molecule (sCD30) has been shown to be released by CD30+ cell in vitro and in vivo. Increased serum levels of sCD30 have been reported for patients with CD30+ ALCL and CD30+ embryonal carcinoma of the testis and were found to correlate with the clinical phase of the disease, i.e. presentation complete remission (CR), relapse.
Components
Aluminium pouch(es) with a Microwell Plate coated with Monoclonal Antibody (murine) to human sCD30, HRP-Conjugate (anti-sCD30 monoclonal (murine) antibody) and Sample Diluent, lyophilized
Aluminium pouch(es) with a human sCD30 Standard curve (coloured)
Wash Buffer Concentrate 20x (phosphate-buffered saline with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Sample Diluent (Use when an external predilution of the samples is needed)
Stop Solution (1M Phosphoric acid)
Adhesive Plate Covers
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