Description: The human sCD40L Instant ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of soluble human CD40 ligand. The human sCD40L Instant ELISA is for research use only. Not for diagnostic or therapeutic procedures.
CD40 is a 48 kD type I glycoprotein and a member of the TNFR superfamily primarily expressed on B cells, macrophages, follicular dendritic cells, endothelial cells, fibroblasts, and at low levels on plasma cells. CD40 is also expressed on a wide range of tumor cells, including non-Hodgkin's and Hodgkin's lymphomas, myeloma and some carcinomas including nasopharynx, bladder, cervix, kidney and ovary.The binding of CD154 (CD40L) on TH cells to CD40 activates antigen presenting cells and induces a variety of downstream effects.
Components
Aluminium pouch(es) with a Microwell Plate coated with Monoclonal Antibody (murine) to human sCD40L, HRP-Conjugate (anti-sCD40L monoclonal (murine) antibody) and Sample Diluent, lyophilized
Aluminium pouch(es) with a human sCD40L Standard curve (coloured)
Wash Buffer Concentrate 20x (phosphate-buffered saline with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Sample Diluent (Use when an external predilution of the samples is needed)
Stop Solution (1M Phosphoric acid)
Adhesive Plate Covers