Description: This Human IL-12p70 High Sensitivity ELISA contains the necessary reagents, from pre-coated plates to standards, buffers and diluents for performing quantitative enzyme-linked immunosorbent assays (ELISA). This ELISA kit is specifically engineered for sensitive and precise measurement of IL-12p70 levels from samples including serum, plasma, and supernatants from cell cultures.
The bioactive form of IL-12 is a 75 kDa heterodimer (IL12p70) comprised of independently-regulated disulfide-linked 40 kDa (p40) and 35 kDa (p35) subunits. The p40 subunit can also form a homodimer which has been shown to be able to bind the IL-12 receptor and thus acts as an IL-12 antagonist. Additionally, the p40 subunit has been found to be expressed in a high excess over p70. IL-12 exerts a variety of biological effects on human T and natural killer cells. Apart from promotion of Th1 development and its ability to promote cytolytic activity it mediates some of its physiological activities by acting as a potent inducer of interferon (IFN) gamma production and the stimulation of other cytokines from peripheral blood T and NK cells.
Components
Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human IL-12p70
Biotin-Conjugate anti-human IL-12p70 monoclonal antibody
Streptavidin-HRP
Human IL-12p70 Standard lyophilized, 400 pg/ml upon reconstitution
Sample Diluent
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Amplification Diluent Concentrate (2x)
Amplification Reagent I*
Amplification Reagent II
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Substrate Solution
Stop Solution (1M Phosphoric acid)
Adhesive Films
* reagent contains ethyl alcohol
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