Description: The human sICAM-2 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human Intercellular Adhesion Molecule-2 (sICAM-2). The human sICAM-2 ELISA is for research use only. Not for diagnostic or therapeutic procedures.
Intercellular Adhesion Molecule-2 (ICAM-2) is a 60 kDa surface glycoprotein that promotes adhesion between immune cells and the vascular endothelium. ICAM-2 is a member of the Ig-superfamily with two immunoglobulin-like extracellular domains. ICAM-2 is much more closely related to the two N-terminal domains of ICAM-1 (34% identity) than either ICAM-1 or ICAM-2 is to other members of the Ig-superfamily, demonstrating the existence of a subfamily of Ig-like ligands that bind the same integrin receptor. ICAM-2 is broadly distributed on hematopoietic cells. On resting lymphocytes, the ICAM-2 expression is several-fold higher than that seen with ICAM-1, while monocytes express equivalent levels of ICAM-1 and ICAM-2. In contrast to ICAM-1, neutrophils stain negative for ICAM-2. Just little or no ICAM-2 staining can be demonstrated on other cell lines, the sole exception being resting vascular endothelial cells, which possess high levels of ICAM-2, and small clusters of cells in lymphoid tissue germinal centres.
ICAM-2 expression is unaffected by a variety of inflammatory cytokines.
Components
Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human sICAM-2
Biotin-Conjugate anti-human sICAM-2 monoclonal antibody
Streptavidin-HRP-Concentrate
Human sICAM-2 Standard lyophilized, 24.00 U/ml upon reconstitution
Streptavidin-HRP Diluent
Standard Buffer Diluent (10x)
Biotin Conjugate Diluent
Wash Buffer Concentrate 200x
Substrate Solution (tetramethyl-benzidine and buffered hydrogen peroxide)
Stop Solution (1.8N H2SO4)
Blue-Dye
Green-Dye
Red-Dye
Adhesive Films
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