Description: The human sPECAM-1 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human sPECAM-1. The human sPECAM-1 ELISA is for research use only. Not for diagnostic or therapeutic procedures.
PECAM-1 is a transmembrane glycoprotein with a molecular weight of approximately 130 kDa, depending on the degree of glycosylation. PECAM-1 is constitutively expressed on all vascular cells and has provided a useful immunohistochemical marker of blood vessels, particularly in the setting of angiogenesis. It has also been found on platelets, monocytes, neutrophils and CD8+ T cells. Bone marrow stem cells and transformed cell lines of the myeloid and megakaryocytic lineage also express PECAM-1. Interestingly, PECAM-1 was also detected on human, mouse and rat solid tumor lines.
Recent studies suggest a role for PECAM-1 in the inflammatory process and leukocyte-endothelial interaction. PECAM-1 is an early and sensitive marker for tumor-induced angiogenesis. Several data have suggested that PECAM-1 may be involved in the process of angiogenesis in a developing vertebrate embryo as well as during metastases formation.
Besides the membrane-bound form of PECAM-1 a soluble form of the molecule exists, which is 5-10 kDa smaller than cell-associated PECAM-1, and contains the cytoplasmic tail. Soluble PECAM-1 was detected in normal human plasma.
Components
Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human sPECAM-1
HRP-Conjugate anti-human sPECAM-1 monoclonal antibody
Human sPECAM-1 Standard, 60 ng/ml
Sample Diluent
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Blue-Dye
Green-Dye
Adhesive Films
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