Description: The human IL-1beta ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human IL-1beta. The human IL-1beta ELISA is for research use only. Not for diagnostic or therapeutic procedures.
IL-1beta is a pro-inflamatory cytokine and is expressed by many cells including macrophage, NK cells, monocytes, and neutrophils. It belongs to the IL-1 family cluster. Associated activities with IL-1 are the induction of Prostaglandin E2 by synovial cells and release of collagenase with resulting destruction of cartilage and bone resorption.
IL-1, has multiple immunological functions including enhancement of IL-2 production by T cells and activation of B-cells (BAF) and thymocytes.
Components
Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human IL-1beta
Biotin-Conjugate anti-human IL-1beta monoclonal antibody
Streptavidin-HRP
Human IL-1beta Standard lyophilized, 500 pg/ml upon reconstitution
Control high, lyophilized
Control low, lyophilized
Sample Diluent
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Blue-Dye
Green-Dye
Red-Dye
Adhesive Films
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